Transgenomic Inc announces issuance of U.S. patent for ICE COLD-PCR Transgenomic announced that the U.S. Patent and Trademark Office has issued a new patent related to its licensed ICE COLD-PCR technology. The patent, titled “Full cold-PCR enrichment with reference blocking sequence”, was assigned U.S. Patent No. 8,623,603 and is set to expire in 2031. The new U.S. patent extends the scope and longevity of the COLD-PCR intellectual property portfolio. The '603 patent covers ICE COLD-PCR methods for enriching low abundance alleles in nucleic acid samples. It is directed in particular to the use of an excess amount of reference blocking sequence in an amplification reaction mixture in order to improve the enrichment efficiency, and reduce cycle time, of full COLD-PCR.